This is the 17222nd most frequent Turkish word.
öldüğünden
"Because (he/she/it) died" or "since (he/she/it) died."
The word 'öldüğünden' is derived from 'ölmek', meaning 'to die'. In this sentence, it indicates a causative or reason clause where the death of his family is the reason for his loneliness.
O, ailesi öldüğünden beri kendini yalnız hissediyor.
Since his family passed away, he has been feeling lonely.
Here 'öldüğünden' refers to the state of the tree being dead. It forms part of a question seeking confirmation.
Bu ağacın öldüğünden emin misiniz?
Are you sure that this tree has died?
In this sentence, 'öldüğünden' is used to express the state or condition of the animals having died, integrated into the speaker's suspicion.